COMPLUCAD^®

Is specially design for piece preservation in Pathologic Anatomy departments. COMPLUCAD HOSPITAL does not contain formaldehyde or any phenolic elements but it’s most outstanding characteristic is the quality of the results obtained. It can be applied by sinking or injecting according to the techniques already described.

When sinking, the piece to be preserved should be immersed for three or four days in a non metallic container with COMPLUCAD HOSPITAL .

If injecting, 70 -80 ml. of COMPLUCAD HOSPITAL should be used per kilogram.

After finishing one of these procedures, the piece can be preserved, without any additional care, in the customary containers or in a Polyethylene Bag.

The need for a fixative process is most common in histology and histopathology. Therefore, it is essential to establish and maintain the cellular structures and components. Although there are many substances with fixative properties, to date there is not a universal fixer. In addition, it is proven that most of the fixative substances are highly toxic.

COMPLUCAD MUÑIZ is a powerful bactericide that preserves histology samples at room temperature. Aside from that, the use of COMPLUCAD MUÑIZ offers many advantages to the histological and anatomopathological fields since this compound is thrombolitic and lypolictic. This effect allows a homogeneous fixation for the tissue samples avoiding blood coagulation and destroys any previous clots. COMPLUCAD MUÑIZ also offers the possibility of restoring elasticity to samples previously treated with other techniques.

COMPLUCAD MUÑIZ shows a powerful fixative effect which later allows the treatment and preservation of different histopathological and histology samples. COMPLUCAD MUÑIZ provides rapid fixations while eliminating toxicity risks for the eyes, skin and respiratory tract.

The methodology employed for a fixative process with COMPLUCAD MUÑIZ is fast and the results are highly satisfactory in optic microscopy, electronic microscopy and inmunihistochemistry.

Dilute 10 parts of COMPLUCAD MUÑIZ in 90 parts of 70% ethanol to obtain the usage solution.

The pieces to be prepared will have an approximate volume of 1 cm³ in case that the pieces are bigger the fixation time will proportionally vary.

FIXATION
After one hour and forty five minutes the piece is fixed. The limits (ranges) for a perfect fixation go from one hour and forty five minutes to four hours.

DEHYDRATION
Once the tissue is fixed, it is subject to increases of ethanol solutions with the following time schedule:

• 1.- Ethanol 70%, soak it three times for 15 minutes.
• 2.- Ethanol 96%, soak it three times for 15 minutes.
• 3.- Ethanol 100% soak it four times for 10 minutes.

Afterwards it would be subject to a 10 minute dip in Toluene, Xylem or Histolemon.

PARAFFIN INCLUSION

• Soak it for one hour in restored paraffin.
• Soak it for one hour in new paraffin.

NOTE:

COMPARATIVE FIXATIONS OF HUMAN TISSUE CARRIED OUT BY THE ANATOMY PATHOLOGY DEPARTMENT OF THE MEDICAL SCHOOL FROM MALAGA UNIVERSITY

PROSTATE

Prostate. Phormol fixation. Dye with hematoxiline-eosine

Prostate. COMPLUCAD fixation. Dye with hematoxiline-eosine

LIVER

Liver. Phormol fixation. Dye with hematoxiline-eosine. .
Liver. COMPLUCAD fixation. Dye with hematoxiline-eosine	Liver. COMPLUCAD fixation. Inmunohistochemist dye with vimentine.

MUCULAR FIBERS

Mucular fibers Phormol fixation. Dye with hematoxiline-eosine	Mucular fibers COMPLUCAD fixation. Dye with hematoxiline-eosine .
Mucular fibers Phormol fixation. Inmunohistochemist dye with actina	Mucular fibers COMPLUCAD fixation. Inmunohistochemist dye with actina

COMPLUCAD MUÑIZ APPLICATION FOR TISSUE FIXING IN OPTIC MICROSCOPY

Epithelium detail, fixed by immersion with COMPLUCAD MUÑIZ and stained with Hematoxiline-eosine.	Paraffin section of a rat kidney, fixed by COMPLUCAD MUÑIZ immersion and stained with Hematoxiline-eosine. The glomerolous is surrounded by the Bowman capsule.
Section of a thin rat skin prepared with blue toludine after fixing it with COMPLUCAD MUŅIZ. Note the presence of the thin corneal stratum. The dermis contains numerous pilocide follicles associate to the sebaceous glands.	Transversal section of the rat oviduct fixed by perfusion with COMPLUCAD MUŅIZ and stained with Hematoxiline-eosine. Note the epithelium surrounded by the collective tissue, smooth muscular and adipose tissue.

COMPLUCAD MUÑIZ APPLICATION FOR TISSUE FIXATION IN IMMUNOHISTOCHEMISTRY

Histology cut in paraffin of muscular coat and muscular smooth fibers of an inmunopositive duodenum for the microfilaments of desmine.

Detail of the pulmonary bronchiole for laminine.